Guiding Protein Aggregation with Macromolecular Crowding
نویسندگان
چکیده
منابع مشابه
Macromolecular crowding
Although you were born and educated in the USA, you've worked in the UK for over 11 years. What are your views on the different ways science is done in the two countries? I've probably been away from the States too long to judge! One thing that does stand out, however, is the difference in postgraduate (PhD) education and training. Most students in the UK get funding for a three-year PhD after ...
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Proteins are neither purified nor diluted inside the living cell. Thus it is indispensable to take into account various interactions between the protein of interest and other macromolecules for understanding the properties of proteins in physiological conditions. Here we focus on excluded volume interactions which are omnipresent in dense or crowded solutions of proteins and macromolecules or "...
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The intracellular environment represents an extremely crowded milieu, with a limited amount of free water and an almost complete lack of unoccupied space. Obviously, slightly salted aqueous solutions containing low concentrations of a biomolecule of interest are too simplistic to mimic the "real life" situation, where the biomolecule of interest scrambles and wades through the tightly packed cr...
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Protein dynamics in cells may be different from those in dilute solutions in vitro, because the environment in cells is highly concentrated with other macromolecules. This volume exclusion because of macromolecular crowding is predicted to affect both equilibrium and kinetic processes involving protein conformational changes. To quantify macromolecular crowding effects on protein folding mechan...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2008
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi8008399